Ketogenic diet aggravates hypertension via NF-κB-mediated endothelial dysfunction in spontaneously hypertensive rats
Y Guo, X Wang, P Jia, Y You, Y Cheng, H Deng, S Luo… - Life sciences, 2020 - Elsevier
Y Guo, X Wang, P Jia, Y You, Y Cheng, H Deng, S Luo, B Huang
Life sciences, 2020•ElsevierAims Ketogenic diet (KD) has been proposed to be an effective lifestyle intervention for
metabolic syndrome. However, the effects of KD on hypertension have not been well
investigated. The present study aimed to investigate the effects and underling mechanisms
of KD on hypertension in spontaneously hypertensive rats (SHRs). Materials and methods
SHRs were subjected to normal diet or KD for 4 weeks, starting at the age of 10 weeks.
Then, the blood pressure and vascular function were assessed. Next, the eNOS expression …
metabolic syndrome. However, the effects of KD on hypertension have not been well
investigated. The present study aimed to investigate the effects and underling mechanisms
of KD on hypertension in spontaneously hypertensive rats (SHRs). Materials and methods
SHRs were subjected to normal diet or KD for 4 weeks, starting at the age of 10 weeks.
Then, the blood pressure and vascular function were assessed. Next, the eNOS expression …
Aims
Ketogenic diet (KD) has been proposed to be an effective lifestyle intervention for metabolic syndrome. However, the effects of KD on hypertension have not been well investigated. The present study aimed to investigate the effects and underling mechanisms of KD on hypertension in spontaneously hypertensive rats (SHRs).
Materials and methods
SHRs were subjected to normal diet or KD for 4 weeks, starting at the age of 10 weeks. Then, the blood pressure and vascular function were assessed. Next, the eNOS expression, inflammatory factors and relative signaling pathway were examined. Human umbilical vein endothelial cells were used to investigate the underlying mechanism account for the effect of ketone on inflammation and eNOS expression.
Key findings
Compared with the normal diet, KD was indicated to aggravate hypertension and impaire endothelium-dependent relaxation in mesenteric arteries of SHRs. eNOS and CD31 expression in mesenteric arteries were also significantly suppressed by KD. In addition, KD markedly increased the activation of NF-κB pathway and the expression of IL1-β and TNF-α. In vitro, results showed that inhibition of NF-κB could rescue the adverse effects of ketone body and TGF-β on eNOS expression and inflammation response.
Significance
Our study indicated that KD impaired endothelium-dependent relaxation in mesenteric arteries and aggravated the development of hypertension in SHRs, suggesting that it should be more cautious to apply KD into clinical application in hypertensive individuals.
Elsevier