Semi-quantitative determination of protein expression using immunohistochemistry staining and analysis: an integrated protocol
AR Crowe, W Yue - Bio-protocol, 2019 - bio-protocol.org
Semi-quantitative IHC is a powerful method for investigating protein expression and
localization within tissues. The semi-quantitative immunohistochemistry (IHC) involves using
software such as free software ImageJ Fiji to conduct deconvolution and downstream
analysis. Currently, there is lack of an integrated protocol that includes a detailed procedure
of how to measure or compare protein expression. Publications that use semi-quantification
methods to quantify protein expression often don't provide enough details in their methods …
localization within tissues. The semi-quantitative immunohistochemistry (IHC) involves using
software such as free software ImageJ Fiji to conduct deconvolution and downstream
analysis. Currently, there is lack of an integrated protocol that includes a detailed procedure
of how to measure or compare protein expression. Publications that use semi-quantification
methods to quantify protein expression often don't provide enough details in their methods …