Understanding the immunopathogenesis of neuroimmunological diseases of the CNS
requires a robust method for isolating and characterizing the immune effector cells that
infiltrate the spinal cord in animal models. We have developed a simple and rapid isolation
method that produces high yields of spinal cord infiltrating leukocytes from a single
demyelinated spinal cord and which maintains high surface expression of key
immunophenotyping antigens. Using this method and the Theiler's virus model of chronic …