[HTML][HTML] Proteomics analysis of A33 immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein …
Exosomes are 40–100-nm-diameter nanovesicles of endocytic origin that are released from
diverse cell types. To better understand the biological role of exosomes and to avoid
confounding data arising from proteinaceous contaminants, it is important to work with highly
purified material. Here, we describe an immunoaffinity capture method using the colon
epithelial cell-specific A33 antibody to purify colorectal cancer cell (LIM1215)-derived
exosomes. LC-MS/MS revealed 394 unique exosomal proteins of which 112 proteins (28%) …
diverse cell types. To better understand the biological role of exosomes and to avoid
confounding data arising from proteinaceous contaminants, it is important to work with highly
purified material. Here, we describe an immunoaffinity capture method using the colon
epithelial cell-specific A33 antibody to purify colorectal cancer cell (LIM1215)-derived
exosomes. LC-MS/MS revealed 394 unique exosomal proteins of which 112 proteins (28%) …